![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
ABSTRACT
Oogonial development of Achlya recurva Cornu (sensu Latham) was studied utilizing the Barka-Anderson and Hugen Borgers modifications of the Gomori reaction. The differentiation of the oogonium was subdivided into stages A-1, A-2, and A-3. Transmission electron microscopy revealed that acid phosphatase reaction product (ACP) was localized primarily within apical vesicles of oogonial protuberances within stage A-1 oogonia. Other sites of ACP within A-1 oogonia included vesicles beneath the oogonial wall and the periphery of the oogonial wall. Numerous vesicles within antheridia and areas of vacuolation such as the oogonial stalk had substantial amounts of ACP. Within the A-2 oogonia, ACP was localized primarily at the periphery of the oogonial wall. In contrast, in the A-3 oogonia ACP was very prevalent within dictyosomes and the granular component of “fingerprint” vacuoles. Alkaline phosphatase reaction product (ALP) was observed in dictyosomes, cytoplasmic vesicles, oogonial walls, “fingerprint” vacuoles, and nuclei of A-3 oogonia as well as extra-cellularly. Possible functions of these enzymes in relationship to sex-organ morphogenesis are discussed.