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ABSTRACT
Antigenic differences associated with myxamoebal clones of different mating types in Didymium iridis and Physarum polycephalum were examined using fluorescent antibodies and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunofluorescence of heteroadsorbed anti-myxamoebal sera showed there is no mating strain-specific activity in either species but the sera were shown to be genus-specific. Intergeneric differences and similarities were revealed in the electrophoretic patterns of the myxamoebal protein extracts from P. polycephalum, D. iridis, and Dictyostelium discoideum. Intraspecific differences were noted in D. iridis.