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ABSTRACT
Mycotrophein, a growth factor required by some biotrophic contact mycoparasites, was isolated from Arthrobotrys musiformis. Mycotrophein was partially purified using a combination of silicic acid and high performance liquid chromatographies. The partially purified compound promoted axenic growth of Stephanoma phaeospora, Gonatobotrys simplex, Calcarisporium parasiticum and Gonatorrhodiella highlei, contact mycoparasites which require this factor for axenic growth. There was essentially no growth-promoting activity with any of the many authentic biochemicals tested. Electron impact mass spectral measurements on the purified mycotrophein indicated only the presence of a mixture of tetra-and pentaethylene-glycol mono-(nonylphenyl) ethers that could possibly act as carriers of the biologically active factor.