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ABSTRACT
Several Ceratobasidium isolates (binucleate Rhizoctonia solani-like fungi), all in anastomosis group 7 (CAG-7), developed basidiospores in axenic agar culture. There was wide variation in reproductive capacities among isolates. Under conditions favorable for sporulation, the most prolific isolate produced 500–1500 × 103 spores/60 mm Petri dish culture in 12 da. Meager sporulation in dark-grown cultures and profuse sporulation in light-grown cultures established that light was not required, although highly stimulatory. Sporulation was abundant on V-8 juice agar, low on cornmeal agar, but absent on potato dextrose, Czapek's, and Mycophil agars. Optimal temperature for sporulation was 24 C. At 28 C, light stimulated formation of basidia, but was inhibitory to basidiospore formation.