![Sample our Environment & Agriculture journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5934/TOC-Subject-Sample-2021-Environment-Agriculture.jpg"})
ABSTRACT
The DNA-specific fluorochrome acriflavin has been used to stain meiotic chromosomes in Neurospora. The acriflavin method is simple and highly reliable. Acriflavin-stained nuclei show good contrast and excellent resolution for detailed chromosome analysis. The nucleolus organizer region can be seen as an attenuated strand embedded within the unstained nucleolus ghost. Since spindles and spindle pole bodies do not fluoresce at all, the method is useful for determining chromosome numbers during condensed division stages in the ascus.