![Sample our Environment & Agriculture journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5934/TOC-Subject-Sample-2021-Environment-Agriculture.jpg"})
ABSTRACT
Eleven specimens of basidiospores of Pisolithus arhizus of various provenances were assayed for their ability to germinate. Three incubation amendments were tested for their basidiospore germination induction value. Two amendments were made in combination, using different seedlings on differently amended media. An additional assay was made using two germination activator compounds. Basidiospores of two specimens, one from South Africa and one from Australia germinated on some of the combination plates of seedlings and amended media. No germination of basidiospores occurred with the activator compounds (1-nonanol and abietic acid).
Intrastrain single-spore colonies were mated to form heterokaryotic colonies and a heterothallic, tetrapolar mating system was determined. Homokaryotic colonies of the four mating types of the two isolates were paired and were found not to be compatible. Basidiospores of the eleven specimens were grouped according to basidiospore spine morphology into three distinct groups. From these results the existence of biological species in P. arhizus is suggested.
