A Method of Detection of Fumonisins in Corn Samples Associated with Field Cases of Equine Leukoencephalomalacia

Ronald D. PlattnerU.S. Department of Agriculture, Agricultural Research Service, Northern Regional Research Center, Bioactive Constituents Research, 1815 North University, Peoria, Illinois61604

William P. NorredU.S. Department of Agriculture, Agricultural Research Service, Richard B. Russell Agricultural Research Center, Toxicology and Mycotoxins Research Unit, P.O. Box 5677, Athens, Georgia30613

Charles W. BaconU.S. Department of Agriculture, Agricultural Research Service, Richard B. Russell Agricultural Research Center, Toxicology and Mycotoxins Research Unit, P.O. Box 5677, Athens, Georgia30613

Kenneth A. VossU.S. Department of Agriculture, Agricultural Research Service, Richard B. Russell Agricultural Research Center, Toxicology and Mycotoxins Research Unit, P.O. Box 5677, Athens, Georgia30613

Robert PetersonU.S. Department of Agriculture, Agricultural Research Service, Northern Regional Research Center, Bioactive Constituents Research, 1815 North University, Peoria, Illinois61604

Darcy D. ShackelfordU.S. Department of Agriculture, Agricultural Research Service, Northern Regional Research Center, Bioactive Constituents Research, 1815 North University, Peoria, Illinois61604

David WeislederU.S. Department of Agriculture, Agricultural Research Service, Northern Regional Research Center, Bioactive Constituents Research, 1815 North University, Peoria, Illinois61604

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ABSTRACT

Fumonisin B₁ and B₂, members of a new class of mycotoxins, were measured in culture material of Fusarium moniliforme MRC826, and in two corn samples associated with field cases of equine leukoencephalomalacia. The compounds were detected by thin-layer chromatography (TLC) with confirmation by liquid secondary ion-mass spectrometry (SIMS) and by gas chromatography/mass spectrometry (GC/MS). Reference standards were isolated from cultures of F. moniliforme on corn. The level of fumonisin B₁ was about 600 mg/kg in the culture material and 150 and 20 mg/kg in the two naturally contaminated samples.

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A Method of Detection of Fumonisins in Corn Samples Associated with Field Cases of Equine Leukoencephalomalacia

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A Method of Detection of Fumonisins in Corn Samples Associated with Field Cases of Equine Leukoencephalomalacia

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