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ABSTRACT
Random amplified polymorphic DNA markers were developed for Cronartium quercuum f. sp. fusiforme, causal agent of fusiform rust disease on pines in the southern United States. Genetic linkages were identified between 103 marker loci in a population of 48 gametothalli and drops of spermatia produced from a single urediniospore culture. A preliminary genetic linkage map with eight linkage groups and 12 linked pairs is presented. Based on the extent of linkage detected among loci, the size of the nuclear genome of C. q. f. sp. fusiforme is estimated to be approximately 5100 recombination units. The 103 marker loci characterized are estimated to provide approximately 40% coverage of the genome. The random amplified polymorphic DNA markers may be of immediate utility in fingerprint identification of laboratory cultures and field isolates for epidemiological studies. Even this preliminary genetic linkage map has great potential for aiding in studies on the host-pathogen interaction in fusiform rust disease and monitoring variation in pathogen populations.