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Abstract
Double-stranded RNA was identified in six isolates of Tilletia indica, the causal agent of Karnal bunt disease of wheat. Double-stranded RNAs ranged from 2.8 to 12.3 kbp in size, similar to sizes reported for double-stranded RNAs from other plant pathogenic fungi. The six isolates revealed diverse double-stranded RNA banding patterns; however, identical patterns were found for two isolates (one from India, one from Mexico). A random-primed cDNA probe generated from the high molecular weight double- stranded RNA segment of isolate PV-18 hybridized only to double-stranded RNA of isolate PV-18 and not to double-stranded RNA of the other five isolates, indicating a high degree of sequence specificity within the double-stranded RNA from at least one of the isolates tested. This is the first report of double-stranded RNA occurring in any species of Tilletia.