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Abstract
The spectrophotometric fluorescein diacetate assay was used to quantify viable mycelial growth of Phanerochaete chrysosporium on Pinus banksiana wood chips. Tests were conducted on inoculated nonsterile and sterile chips with noninoculated nonsterile and sterile chips as control. Additionally, some chips were compressed to make nutrients more readily accessible by mechanically rupturing the wood parenchyma cells. Comparisons were made of chips heavily colonized with P. chrysosporium against chips with only scattered mold mycelium to test the effectiveness of the fluorescein diacetate assay for this particular species. It was found that P. chrysosporium did not produce significant hydrolysis of fluorescein diacetate as compared to other resident microflora.