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Abstract
Endophytic Acremonium isolates were obtained from 43 of 50 plants of Stipa robusta grown from seed collected from 10 locations in New Mexico and Colorado. Hyphae in the lower 2–3 cm of seedlings grown aseptically from infected seed were intercellular, about 2 μm in diameter, mostly straight. Coarse sinuous or convoluted hyphae were produced in culture to some extent by many isolates and conidia were seen in all cultures. Observations of fungal structures were easily made on water agar. When first cultured, mean conidial lengths of individual isolates varied from 5.9 to 8.7 μm. Considerable variation in conidial shapes occurred and average spore lengths of most isolates did not conform to described species, but were intermediate between A. starii and A. coenophialum. The most common type of spore was too short for A. coenophialum and too long for A. starrii, and no isolate even faintly resembled A. chisosum. Spores of most isolates were dead in cultures 60 days old at 22 C. Serological analysis demonstrated the relatedness of the isolates to endophyte isolates from a broad range of other grasses.
