Monoxenic culture of the intraradical forms of Glomus sp. isolated from a tropical ecosystem: a proposed methodology for germplasm collection

Abstract

Five arbuscular mycorrhizal (AM) fungi, isolated from the rhizosphere of banana and sugar cane, were successfully cultured in vitro in association with genetically transformed roots of carrot. The intraradical forms of the fungi as mycorrhizal root pieces and single isolated vesicles constituted excellent sources of inoculum for the establishment of in vitro cultures and for the continuous culture of the species. Several thousand fungal propagules were obtained for the two G. versiforme species, G. intraradices and to a lesser extent for G. fasciculatum whereas few spores were obtained for G. macrocarpum. This coculture system appeared suitable for the establishment of in vitro collections of AM fungal strains.

Key Words:

• arbuscular mycorrhizal fungi
• continuous culture
• fungal library
• Ri T-DNA transformed root
• vesicle