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Abstract
Two new species of harpellid gut fungi, Smittium fecundum and Stachylina robusta, were found living in larvae of Psectrocladius sp. (Diptera: Chironomidae) in a high-altitude kettle pond in the Rocky Mountains of Colorado. A third species of Harpellales, Smittium mucronatum, previously known only from Psectrocladius sordidellus in southern France, was found co-inhabiting the hindguts of the Colorado Psectrocladius larvae. Several axenic isolates of S. fecundum were obtained.