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Abstract
Mycelial isolates of tropical wood-inhabiting basidiomycetes are not normally considered candidates for preservation by lyophilization because these fungi do not form resistant propagules. This study investigated alternative methods of lyophilization of such mycelial isolates. Media components, freezing rates, dehydration and rehydration, and lyoprotectants were assessed for effects on viability. Results showed that these fungi can survive lyophilization. No noticeable changes in viability, growth rates, or morphological and biochemical characteristics occurred after lyophilization.