https://orcid.org/0000-0002-2558-3404
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ABSTRACT
The sclerotia of Pachyma hoelen are one of the traditional Chinese medicines and foods that are widely used in East Asian countries. The strains used for cultivation showed bad performance in recent years, and breeding of superior strains has become increasingly important for this fungus. Nevertheless, the mating system and life cycle of P. hoelen were still ambiguous. In this study, the methods for distinguishing between homokaryotic offspring with different mating types were established, as well as confirmation of strain hybridization based on allelic polymorphism at a locus of the rpb2 gene. The bipolar mating system was confirmed according to the mating results of homokaryotic SSIs. The fact that heterokaryotic parents produce homokaryotic meiospores proves that the life cycle is heterothallic. Combining scanning electron microscope observation and DAPI (4′,6-diamidino-2-phenylindole) fluorescent staining of hymenium and basidiospores in situ and ex situ, nuclear migration pattern from basidia to spores was revealed. The heterothallic life cycle was verified, revised, and supplemented step by step. This is the first report of systematic research on the mating system, life cycle, and outcrossing of homokaryotic offspring in P. hoelen. It will be helpful for the biological research, strain improvement, and development of the P. hoelen industry.
ACKNOWLEDGMENTS
We thank the anonymous reviewers and editors for their helpful comments and suggestions.
DISCLOSURE STATEMENT
No potential conflict of interest was reported by the author(s).
FUNDING
This study was supported by the National Key Research and Development Program of China (no. 2017YFC1703003), and Science and Technology Support Plan of Guizhou Province in 2021 (Qian Ke He Fundamental No. [2021] 249).
SUPPLEMENTARY MATERIAL
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