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Abstract
Fractionation of 0.3M LiOH soil extracts with added 32P myoinositol hexaphosphate, by the anion-exchange chromatography procedures used in previous studies of soil organic phosphorus, showed that recovery of 32P activity varied from 8–65 percent depending on the anion-exchange resin used.
Isotope dilution analysis gave values for the inositol hexaphosphate content of 0.3M LiOR extracts of four topsoils, corrected for low recoveries during fractionation. Because of low resolution of the resin chromatography methods, myo-inositol penta phosphate and the pentaand hexa-phosphates of other inositol isomers present in the soil extracts are included in these values.
From 86–100 percent of the organic phosphorus extracted from the topsoils was accounted for when the values for inositol polyphosphates obtained by isotope dilution were combined with values for the other fractions obtained by direct phosphorus analysis. Inositol phosphates represented 40–72 percent of the extracted soil organic phosphorus, humic acid-associated phosphorus 19–44 percent, and a fraction not yet characterised 3–8 percent.