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Endosperm transplants were used to aid the in vitro culture of young Lotus embryos. Three tetraploid hybrids of 4x L. pedunculatus × 4x L. tenuis were obtained. Three tetraploid hybrids of 4x L. tenuis × L. corniculatus were grown from full-term embryos on a simple medium. Hybridity for all tetraploids was confirmed by morphological studies and, where appropriate, by the presence of hydrocyanic acid, flavanol polymers (“tannins”), and patterns of phosphatase and peroxidase isozymes.
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