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Abstract
Methods for the in vitro propagation of complete Solanum laciniatum Ait. plants from callus, leaves, shoots, and apical meristem tips are described. Callus proliferation occurred on Murashige & Skoog medium (MS) supplemented with 10-6M 6-benzylaminopurine (BAP) and 10-6M β-naphthoxyacetic acid. Maximum shoot organogenesis from this callus occurred on MS + 10-5M BAP. Adventitious shoot proliferation from both leaves and shoots was also possible on MS + 10-5M BAP, as was the development of apical meristem tips (0.3– 0.5 mm long) into shoots. Cultured shoots readily formed roots when subcultured on to MS medium without plant growth regulators and were successfully transferred to a greenhouse.