Assessing sperm membrane viability using flow cytometry in farmed New Zealand giant kokopu Galaxias argenteus

Johnston PearceInstitute for Applied Ecology New Zealand, Auckland University of Technology, Auckland, New Zealand

Armagan SabetianInstitute for Applied Ecology New Zealand, Auckland University of Technology, Auckland, New ZealandCorrespondence[email protected]

Liz-Marie KeyserInstitute for Applied Ecology New Zealand, Auckland University of Technology, Auckland, New Zealand

Sheng WangInstitute for Applied Ecology New Zealand, Auckland University of Technology, Auckland, New Zealand

Jun LuInstitute for Applied Ecology New Zealand, Auckland University of Technology, Auckland, New Zealand;College of Life and Marine Sciences, Shenzhen University, Shenzhen, People’s Republic of China;School of Interprofessional Health Studies, Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland, New Zealand

ABSTRACT

We employed flow cytometry to assess plasma membrane viability of sperm cells in farmed giant kokopu Galaxias argenteus, an endemic amphidromous New Zealand fish. Specifically, the variables of fish size (TL mm) and weight (grams) were assessed against sperm viability. We found no significant difference (P < .05) in sperm viability when compared across size and weight distribution of G. argenteus. This study shows that flow cytometry is an effective, accurate and rapid method for analysing sperm viability in G. argenteus.

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Acknowledgements

We would like to thank Paul Decker from New Zealand Premium Whitebait Limited for access to their facility and also supplying us with specimens for this study.

Disclosure statement

No potential conflict of interest was reported by the authors.

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Assessing sperm membrane viability using flow cytometry in farmed New Zealand giant kokopu Galaxias argenteus

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Assessing sperm membrane viability using flow cytometry in farmed New Zealand giant kokopu Galaxias argenteus

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