Differentiating Epithelial Cells From Leukocytes in Urine

Abstract

When leukocytes react with peroxidase, granules in the cytoplasm stain dark blue, but epithelial cells from the urethra, bladder, ureters and kidneys show no reaction. By using a benzidine peroxidase mixture, one can stain smears of urine sediment in two minutes, differentiate leukocytes from epithelial cells, and store the slides permanently.