A Simplified Procedure for Isolation of Golgi Apparatus from Rat Liver

D. James Morré Department of Botany and Plant Pathology and Department of Biological Sciences, Purdue University, Lafayette, Indiana, 47907

R. D. Cheecham Department of Botany and Plant Pathology and Department of Biological Sciences, Purdue University, Lafayette, Indiana, 47907; Department of Biology, Minnesota State University, Marshall, Minnesota

S. E. Nyquist Department of Botany and Plant Pathology and Department of Biological Sciences, Purdue University, Lafayette, Indiana, 47907; Charles F. Kettering Research Laboratory, Yellow Springs, Ohio

Ludmila Ovtracht Laboratoire de Biologie Cellulaire, PCEM, Section C, Faculté des Sciences de Paris, 94, Ivry-sur-Seine, France

Abstract

A simplified method is described for isolation of Golgi apparatus from rat liver. The procedure provides high yields of intact, enzymatically active preparations relatively free from contamination by other cell components. When large volume, swinging bucket rotors are employed, it is possible to process livers from 10–12 rats in a single run of less than 2 hours.

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A Simplified Procedure for Isolation of Golgi Apparatus from Rat Liver

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A Simplified Procedure for Isolation of Golgi Apparatus from Rat Liver

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