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Abstract
Reversed-phase chromatography has been applied to the rapid separation of E. coli 5S rRNA and has also been adapted for use on an analytical scale for the rapid (about 30 min) separation of small quantities (0.1 Ap260 unit) of l6s and 23S rRNAs. Compared to other techniques, this nondestructive method is faster, more sensitive, and gives better resolution.