Three Modifications of the Dodecyl Sulfate Method of Kay et al. for Isolating DNA from Calf Thymus

Abstract

Two rapid methods for the isolation of calf thymus DNA are described which are modifications of the method of Kay et al. ¹ for isolating DNA. The principal changes introduced are that degelation of the DNA-nucleo-protein gels is accomplished rapidly by the use of mild shear or by pH adjustment in the presence of isopropanol instead of by the more time-consuming autolytic proteolysis used in the original method.