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Abstract
A procedure is described for extracting cardiac glycosides and their aglycones from dried leaf powder of Digitalis purpureaL. by a water-ethanol gradient elution followed by Soxhlet extraction. Milligram amounts of pure digitoxin had been added to the leaf powder for studying its effects on the solubilities and removal of interfering plant pigments and on the recovery of steroidal substances by thin-layer chromatography. Definite effects of added digitoxin on the turbidity of plant extracts and on plausible com-plexing reactions are described, some of which proceed parallel to aging effects of plant extracts.