Purification of Udp-Glucose Dehydrogenase by Hydrophobic and Affinity Chromatography

C. R. Geren Department of Biochemistry, University of Kansas Medical Center, Kansas City, Kansas, 66103

C. M. Olomon Department of Biochemistry, University of Kansas Medical Center, Kansas City, Kansas, 66103

D. C. Primrose Department of Biochemistry, University of Kansas Medical Center, Kansas City, Kansas, 66103

K. E. Ebner Department of Biochemistry, University of Kansas Medical Center, Kansas City, Kansas, 66103

Abstract

UDP-glucose dehydrogenase was purified from an extract of calf liver acetone powder by sequential chromatography on norleucine-agarose, Type 2, agarose-hexane-AMP, and UDP-hexanolamine-Sepharose. The overall yield of the highly purified enzyme was 80 percent and the subunit molecular weight was 52, 000.

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Purification of Udp-Glucose Dehydrogenase by Hydrophobic and Affinity Chromatography

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Purification of Udp-Glucose Dehydrogenase by Hydrophobic and Affinity Chromatography

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