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Preparative Biochemistry Volume 9, 1979 - Issue 3
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Original Articles

A Rapid Purification Procedure for L-Asparaginase from Vibrio Succinogenes

Abraham Abuchowski Department of Biochemistry, Rutgers University, New Brunswick, N. J., 08903; Department of Zoology and Physiology, Rutgers University, Newark, N. J., 07102
,
David Kafkewitz Department of Biochemistry, Rutgers University, New Brunswick, N. J., 08903; Department of Zoology and Physiology, Rutgers University, Newark, N. J., 07102
&
Frank F. Davis Department of Biochemistry, Rutgers University, New Brunswick, N. J., 08903; Department of Zoology and Physiology, Rutgers University, Newark, N. J., 07102
Pages 205-211 | Published online: 05 Dec 2006
 
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Abstract

A simple procedure has been developed for the purification of L-asparaginase from Vibrio succinogenes. Only two steps of ion-exchange chromatography are required. A higher yield and higher specific activity are obtained than previously reported.

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