Chemical Synthesis of Nucleoside-λ- [³²p]Triphosphates of High Specipic Activity

A. Janecka Department of Biochemistry and Department of General Chemistry, Institute of Physiology and Biochemistry, Medical Academy of Lódź

H. Panusz Department of Biochemistry and Department of General Chemistry, Institute of Physiology and Biochemistry, Medical Academy of Lódź

J. Pankowski Department of Biochemistry and Department of General Chemistry, Institute of Physiology and Biochemistry, Medical Academy of Lódź

W. Koziolkiewicz Department of Biochemistry and Department of General Chemistry, Institute of Physiology and Biochemistry, Medical Academy of Lódź

Abstract

A simple chemical procedure for the preparation of four common ribonucleoside 5-λ-[32p] triphosphates of high specific activity (up to 10 Ci/mmole) based on the condensation of orthophosphoric acid with the corresponding nucleoside 5-diphosphate in the presence of ethyl chloro-formate as well as the methods of purification and identification of the products are described.

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Chemical Synthesis of Nucleoside-λ- [³²p]Triphosphates of High Specipic Activity

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Chemical Synthesis of Nucleoside-λ- [32p]Triphosphates of High Specipic Activity

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Chemical Synthesis of Nucleoside-λ- [³²p]Triphosphates of High Specipic Activity