Preparation of L- and D-[1-¹⁴C]Lysine by the Resolution of DL-[1-¹⁴C]Lysine

Abstract

L-[1-¹⁴ C]lysine was prepared using a technique based on the differential solubility of the diastereomeric salts of DL-lysine with L-glutamic acid. D-[l-¹⁴ C]llysine was prepared by the enzymatic removal of residual L-[l-¹⁴ cl]ysine from the reaction mixture remaining after the preparation of L-[1-¹⁴¹- C]lysine. The products were tested with L-lysine decarboxylase and by column chromatography. L-[l-¹⁴C]lysine was obtained in 60% yield with a steroisomeric purity of 95.1%, and D-[l-¹⁴ cllysine was obtained in a slightly higher yield with a steroisomeric purity of 97.5%.