Preparative Scale Fractionation of Escherichia coli Ribosomal RNA and Transfer RNA on Sepiiarose 6B

Abstract

Total Escherichia coil RNA has been fractionated on Sepharose 6B in 0.1 M ammonium acetate, pH 5.0. The elution order was 23S rRNA, 16S rRNA, SS rRNA, and tRNA, which is in contrast with the reported elution order of eukaryotic RNA, chromatographed under similar conditions. tRNA was obtained in two regions, well separated from the high molecular weight rRNA but with a slight contamination of 5S rRNA. The capacity is at least 40 A₂₆₀ units of RNA per ml of gel.