Buffer Electrofocusing of Thyroid Proteins

Darrell A. Daglish Immunopathology Research Unit, Medical Research Council of New Zealand, University of Otago Medical School, Dunedin, New Zealand

Milton T. W. Hearn Immunopathology Research Unit, Medical Research Council of New Zealand, University of Otago Medical School, Dunedin, New Zealand

Andrew J. Paterson Immunopathology Research Unit, Medical Research Council of New Zealand, University of Otago Medical School, Dunedin, New Zealand

Ross L. Prestidge Immunopathology Research Unit, Medical Research Council of New Zealand, University of Otago Medical School, Dunedin, New Zealand

Peter G. Stanton Immunopathology Research Unit, Medical Research Council of New Zealand, University of Otago Medical School, Dunedin, New Zealand

Abstract

The preparative buffer electrofocusing of a variety of thyroid related proteins is described. The study shows that selective electrofocusing of these proteins on granulated gels can be readily achieved with buffer systems of defined chemical composition. Specific proteins were recovered in satisfactory yields (30-70%) with sample loadings in the range 25-500mg.

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Buffer Electrofocusing of Thyroid Proteins

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Buffer Electrofocusing of Thyroid Proteins

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