Purification of Rat Liver Microsomal Trans-2-Enoyl-CoA Reductase

Abstract

It is well known that fatty acid chain elongation involves four steps : (a) condensation of a primer with malonyl-CoA to form the β-ketoacyl CoA; (b) reduction of the resulting β-ketoacyl CoA to a secondary alcohol, β-hydroxyacyl CoA; (c) dehydration of the alcohol to form trans-2-enoyl CoA; and (d) reduction of the trans-2-enoyl CoA to give an acid 2 carbon atoms longer than the primer. ^{1, 2} The enzyme involved in step (d) is trans-2-enoyl-CoA reductase. The trans-2-enoyl-CoA reductase can perform the reduction of trans-2-enoyl-CoA, in the presence of a reduced pyridine nucleotide, to the saturated product. Trans-2-enoyl-CoA reductase can utilize either NADH or NADPH⁴ as a hydrogen donor.