Quantification and Gel Electrophoretic Analysis of Proteins Extracted from Vascular Endothelial Cells

Abstract

The inner walls of tubular organs and body cavities are lined with a sheet composed of cells which control the passage through the walls and thus are of considerable physiological and biochemical interest. They are difficult to prepare but their content can be extracted and analysed, using laminar extraction and sensitive bioluminescence methods. This has been shown for the endothelial cells which line the interior of blood vessels. Although it is dealing with small amounts of material, only a minor part of it is consumed in the analyses. The bioluminescence measurements can thus be combined with other analytical procedures such as protein assay and electrophoresis. The possibilities of extending the biochemical information is of interest in the efforts to clarify the pathogenesis of vascular diseases.