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Original Article

Primary isolation of Cryptococcus neoformans from pigeon excreta on three media

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Pages 165-167 | Published online: 09 Jul 2009
 

Abstract

Ninety-two pigeon fecal samples assayed for Cryptococcus neoformans on plates of Littman, Sabouraud's dextrose agar and thistle seed (Guizotia abyssinica) medium yielded 45, 27, and 19 isolates, respectively. The superiority of Littman medium was attributed to better suppression of bacterial and saprophytic fungal growth and to a distinct blue-grey color of C. neoformans which facilitated its colonial identification. Although saprophytic overgrowth was less frequent on thistle seed medium than on Sabouraud's dextrose agar, some strains of C. neoformans failed to produce brown pigmentation at an early date. Also, in areas of the plates where some rapidly growing saprophytes appeared, C. neoformans failed to assume the tan or brown color, possibly because the saprophytes had destroyed the pigment producing substance.

Zusammenfassung

92 Taubenkotproben, auf Littman's Medium, Sabouraud-Glucose-Agar und Negersaat (Guizotia abyssinica)—Medium auf Cryptococcus neoformans untersucht, ergaben 45, 27 bzw. 9 positive Befunde. Die Uberlegenheit von Littman's Medium wurde der besseren Unterdrückung des Wachstums von Bakterien und saprophytischen Pilzen zugeschrieben, ferner der deutlichen Blaufärbung von C. neoformans-Kolonien, wodurch deren Identifizierung er leichtert wurde.

Obwohl saprophytisches Überwuchern auf Negersaat-Medium weniger häufig war, als auf Sabouraud-Glucose-Agar, zeigten einige Stämme von C. neoformans im Frühstadium keine braune Pigmentierung.

Auch nahm C. neoformans in Arealen der Platten, wo einige schnell wachsende Saprophyten erschienen, keine hellbraune oder braune Farbe an, möglicherweise, weil die Saprophyten die pigment-bildende Substanz zerstört hatten.

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