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Original Article

The assay of delayed hypersensitivity to ribosomal proteins from Nocardia

, &
Pages 147-151 | Published online: 09 Jul 2009
 

Abstract

The biological properties of ribosomal proteins of Nocardia asteroides and N. brasiliensis were studied. These proteins elicited delayed-type hypersensitivity in Nocardia-sensitized guinea-pigs, as well as inhibition of macrophage migration in the peritoneal exudate obtained from the sensitized animals. The delayed reactivity was heat resistant but it was destroyed by treatment with pronase.

Resumen

Se aislaron proteínas ribosomales de Nocardia asteroides y Nocardia brasiliensis y se estudiaron sus propiedades biolóbicas. Estas proteínas pusieron de manifiesto una hipersensibilidad de tipo tardío en cobayos sensibilizados con Nocardia, tanto por pueba cutánea como por inhibición de la migración de los macrófagos obtenidos de la cavidad peritoneal de estos últimos. La respuesta fué mayor siempre que se uso el antigeno homólogo. La actividad inflamatoria de estas proteïnas fué resistente al calor, sin embargo, fué destruída por el tratamiento enzimático con pronasa.

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