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Original Article

The morphology of colony variants of three species of Candida

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Pages 274-279 | Published online: 09 Jul 2009
 

Abstract

The colonies of 12 isolates of 3 Candida spp. with variant colony forms were studied by scanning and transmission electron microscopy. Small colonies were formed by 4 isolates each of C. albicans and C. parapsilosis and by 1 of C. tropicalis. These had an abnormally high proportion of degenerate yeast cells with an associated increase in granular cytoplasmic material intercellularly. The increased matrix in these small colonies formed a thick superficial coat over the organisms. Rough colonies were formed by 1 isolate each of C. albicans, C. tropicalis and C. parapsilosis. The convoluted regions of these colonies contained many pseudohyphal cells but few degenerate cells and little granular or fibrillar material in their intercellular matrices. The shape of colonies of Candida spp. may be altered by variations in the viability or the morphology of the organisms.

Resumen

Las colonias de 12 aislamientos de 3 especies de Candida que mostraron formas variantes, fueron estudiadas por microscopía electrónica de superficie (scanning) y de transmisión. Aparecieron colonias pequeñas, a partir de 4 aislamientos de C. albicans y C. parapsilosis y de l de C. tropicalis, las cuales contenían una proporción anormalmente alta de microorganismos degenerados, con un aumento en el material granular citoplasmático intercelular. El aumento de la sustancia intercelular (matrix) en estas colonias pequeñas, determinaba la formación de una capa gruesa sobre la superficie de los microorganismos. Las colonias rugosas estaban representadas por 1 cepa de C. albicans, C. tropicalis y C. parapsilosis. Las zonas rugosas de estas colonias contenían muchos microorganismos como pseudohifas, pero muy pocos degendos y escoase material granular o fibrilar en sus matrices intercelulares. La forma de las colonias de Candida spp. puede ser modificada por las variaciones en la viabilidad o morfología de los microorganismos.

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