Abstract
The carboxilic activity of giant cells of human granuloma produced by P. brasiliensis was studied. The enzymatic activity was revealed by reddish-brown, purple red, and indigo-blue cytoplasmic precipitate, using the substrates alpha-naphthyl-acetate, naphthol-AS acetate and 5-bromo-4-chloro-indoxyl-acetate respectively.
The giant cells were intensely positive in all cases studied. We believe this esterasic activity is related to the lytic, lisosomic activity of the macrophages and giant cells in response to the activity by the P. brasiliensis in tissue.
Resumen
Se estudia la actividad carboxílica de célules gigantes del granuloma humano producido por el Paracoccidioides brasilienis. La actividad enzimática se revela por precipitado rojo parduzco, rojo púrpura y azul indigo usando los substratos α-naphthyl-acetate, naphthol-AS-acetate and 5-bromo-4-chloroindoxyl respectivamente.
Las células gigantes se observaron intensamente positivas en todos los casos estudiados. Se cree que esta actividad esterésica está relacionada a la actividad lítica, lisosómica del macrófago y por ende de la célula gigante ante la agresión producida en el tejido por el P. brasiliensis.