5
Views
3
CrossRef citations to date
0
Altmetric
Original Article

Lipolytic enzymes of Trichophyton rubrum

&
Pages 313-323 | Published online: 09 Jul 2009
 

Abstract

Trichophyton rubrum cells contain lipase, phospholipases A and B and acyl CoA lysolecithin acyl transferase activities. This dermatophyte excretes lipase and phospholipase A into the growth medium when cultivated in Sabouraud's broth. Extracellular lipase has optimum activity at pH 8.0 whereas the intracellular lipase is maximally active at pH 8.0 whereas the intracellular lipase is maximally active at pH 7.0. The optimum pH of phospholipase A and B activities which are localized in 15000 g sedimentable cell fragments are 7.0 and 6.0 respectively. Supernatant obtained after removal of 1,005,000 g sedimentable fragments from cell extract contains acyl CoA lysolecithin acyl transferase which requires ATP, CoA, Mg2+ and an unsaturated fatty acid for its activity.

Zusammenfassung

Trichophyton rubrum zelle enthalten Aktivität der Lipase, Phospholipase A und B und Acyl CoA Lysolecithin Acyl transferase. Diese Dermatophytien scheiden Lipase und Phospholipase A zur Wachstummitte aus wenn diese in Sabouraud's Fleischbrühe kultiviert ist. Lipase der Außerzello hat höchst Aktivität um pH 8·0, während Lipase der innerzelle höchst Aktiv um pH 7·0 ist. Die günstige pH der phospholipase A und B Aktivität, die in 15000 g ablagarbar zellbruchstücke lokalisiert sind, sind bzw. 7·0 und 6·0 Supernatant der nach dem Umzug der 105000 g ablagarbar Bruchstücke aus dem Zellauszug erhalt ist, enthälten Acyl CoA Lysolecithin Acyl transferase. Es effordert ATP, CoA, Mg und eine unsätigte Fett-Saüre für seine Aktivität.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.