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Original Article

Growth of Trichophyton mentagrophytes on individual amino acids

, &
Pages 299-303 | Published online: 09 Jul 2009
 

Abstract

Trichophyton mentagrophytes was tested for its ability to utilize individual amino acids as a source of carbon and nitrogen in basal medium containing 0·4 mM magnesium sulphate (0·1 g/l) in 0·05 M potassium phosphate buffer at pH 6·5. Growth was quantitated by measurement of both dry weight and fungal protein. Seventeen naturally occurring amino acids supported growth, serving as a source of both carbon and nitrogen. Seven amino acids failed to support growth under these conditions; however three of these could be metabolized for nitrogen, but not for carbon.

Resúmen

Hemos ensayado la abilidad de Trichophyton mentagrophytes para utilizar un número de aminoácidos como una fuente de carbono y nitrógeno en un medio básico que contiene 0·4 mM sulfato de magnesio (0·1 g/l) en una solución buffer de fosfato de potasio 0·05 M con un pH de 6·5. El crecimiento de este organismo fue medido usando como parámetros el peso seco y el contenido proteico. Nuestros resultados muestran que este hongo puede usar diecisiete aminoácidos naturales como proveedores de ambos elementos, carbono y nitrógeno. Sin embargo, bajo estas condiciones experimentales, T. mentagrophytes no. pudo utilizar para crecimiento otros siete aminoácidos estudiados; tres de estos, sin embargo fueron usados por su nitrógeno pero no por su carbono.

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