Abstract
Blastospores of Candida albicans, grown in YM broth (Difco, pH 6·0), were treated with tannic acid as part of a fixation schedule with glutaraldehyde and osmium tetroxide. The results obtained were compared to routine fixation procedures employing glutaraldehyde-osmium tetroxide and potassium permanganate, as well as several schedules using various combinations of the aforementioned fixatives. Glutaraldehyde-osmium tetroxide-tannic acid fixed cells had several morphological structures accentuated, including sharply delineated cytoplasmic and organelle membranes, a detailed cytoplasmic matrix, and an intensified layered cell wall. Tannic acid has been found to react as a mordant between osmium tetroxide and lead citrate, forming a “stabilizing matrix” within the cells. Blastospores appeared to have an improved comprehensive ultrastructural appearance, a result of the tannic acid-matrix preserving cellular components for electron microscopy.
Resumen
Blastospores de Candida albicans ATCC 18804 fueron crecidos en caldo de YM (Difco, pH 6·0) y fueron tratados con acido tanico con respeto a fijación con glutaraldehyde y osmium tetroxide. Cacodylate-HCl fue usade para puffer todos los figadores. Los resultados obtenidos fueron comparados a procedimientos rutinos de fijación utilizando glutaraldehyde-osmium tetroxide y/o permanganate de potasio. Las extraordinarias facciones de les células tratada por acido de tanico parece ser una mejor apariencia ultra-estructural de los blastosporos con mejor contraste de ribosomas, delineción clara de las membranes de cytoplasma y mejor contraste de la region fibrilar exterior de la pared de la célula. PH de al solución de acido tanico fue importante en la manera de resolver y apreciar de la region exterior de la pared de la célula.