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Original Article

Production of specific antibody to Cryptococcus neoformans by hybridomas in vitro

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Pages 157-160 | Received 29 Sep 1980, Accepted 18 Jan 1981, Published online: 09 Jul 2009
 

Abstract

Cells of spleen homogenates from sensitized BALB/c or C57BL/6J mice were fused with cells of a mouse myeloma line (P3X63Ag8 or SP2/0 Ag 14) using polyethylene glycol. Nine of 40 hybridomas from a BALB/c, P3X63Ag8 cross produced anti-cryptococcal antibody. Antibodies of at least four different specificities were identified.

Résumé

Zellen von Milzhomogenaten aus sensibilisierten Balb/c oder C57BL/6U Mäusen wurder unter Verwendung von Polyäthylenglykol mit Zellen einer Maus Myeloma Zellinie (P3X63Ag8 oder SP2/0 Ag 14) fusioniert. 9 von 40 Hybridoma aus einer Fusion von Balb/c- und P3X63Ag8-Zellen produzierten anti-Cryptococcus Antikörper. Antikörper mit zumindest vier verschiedenen Spezifitäten wurden identifiziert.

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