Serum lysophosphatidic acid concentrations measured by dot immunogold filtration assay in patients with acute myocardial infarction

X. Chen Division of Biochemistry, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, BeijingCorrespondence[email protected]

X. Y. Yang Ministry of Education Key Laboratory of Bioorganic Chemistry & Molecular Engineering, Department of Chemical Biology, College of Chemistry, Peking University, Beijing, P. R. China

N. D. Wang Division of Biochemistry, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing

C. Ding Ministry of Education Key Laboratory of Bioorganic Chemistry & Molecular Engineering, Department of Chemical Biology, College of Chemistry, Peking University, Beijing, P. R. China

Y. J. Yang Division of Biochemistry, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing

Z. J. You Ministry of Education Key Laboratory of Bioorganic Chemistry & Molecular Engineering, Department of Chemical Biology, College of Chemistry, Peking University, Beijing, P. R. China

Q. Su Division of Biochemistry, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing

J. H. Chen Ministry of Education Key Laboratory of Bioorganic Chemistry & Molecular Engineering, Department of Chemical Biology, College of Chemistry, Peking University, Beijing, P. R. China

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Abstract

In this study the relation between lysophosphatidic acid (LPA) and myocardial infarction was investigated, the typical and simplified methods for measuring serum LPA concentration by dot immunogold filtration assay (DIFA) based on a polyclonal antibody to LPA were developed, and serum LPA concentrations were measured in 31 patients with acute myocardial infarction (AMI) and 12 controls (blood donors) by DIFA. Serum LPA levels were raised more than twofold 8 h after the onset of AMI. Maximal elevation (10.43 mg/L) was found at 48–72 h following onset and remained higher than the control concentration (1.66 mg/L) 7 days after AMI. The rise in serum LPA concentration in AMI patients suggests that LPA might be involved in AMI‐related pathophysiology in the cardiovascular system. The simplified DIFA developed in the present study for measuring serum LPA concentration is convenient and highly sensitive.

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Serum lysophosphatidic acid concentrations measured by dot immunogold filtration assay in patients with acute myocardial infarction

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Serum lysophosphatidic acid concentrations measured by dot immunogold filtration assay in patients with acute myocardial infarction

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