9
Views
4
CrossRef citations to date
0
Altmetric
Original Article

Inhibition of chemiluminescence by carvedilol in the cell‐free system, whole human blood and blood cells

, , , , &
Pages 55-64 | Received 16 Feb 2004, Accepted 24 Aug 2004, Published online: 08 Jul 2009
 

Abstract

Carvedilol inhibits luminol‐enhanced chemiluminescence of reactive oxygen metabolites in vitro. In this study it was found that, in the cell‐free system, carvedilol dose‐dependently decreased chemiluminescence in the following ranking order of radicals: hydroxyl radical>hydrogen peroxide>superoxide radical. The inhibition of myeloperoxidase was significant with carvedilol concentrations of 10 and 100 µmol/l and manifested in the concentration‐dependent shift of chemiluminescence peaks to the right. In whole blood, carvedilol in concentrations of 10 and 100 µmol/l significantly inhibited chemiluminescence induced by both receptor‐bypassing stimuli (A23187, PMA) and receptor‐operating stimuli (fMLP, OpZ). Carvedilol dose‐dependently inhibited chemiluminescence of isolated human polymorphonuclear leucocytes in the ranking order of stimuli: A23187>OpZ>fMLP. In the presence of blood platelets, carvedilol did not substantially change chemiluminescence induced by fMLP and OpZ, while it was much more effective on chemiluminescence stimulated with calcium ionophore A23187. This could be the result of the supportive effect of serotonin liberated from platelets by A23187.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.