High-performance liquid chromatographic determination of creatinine

Abstract

Ekelund S, Påby P. High-performance liquid chromatographic determination of creatinine. Scand J Clin Lab Invest 1991; 51: 67–71.

A high-performance liquid chromatography (HPLC) method for creatinine has been developed. The method is based on ion-pair chromatography. The method is precise and easy to perform. Recoveries from enriched sera were from 98–105% with a mean of 100.5%. At 28 μmol l^-1, 52 μmol l^-1 and 487 μmol l^-1 within-series standard deviations of 2 μmol l^-1, 2 μmol l^-1 and 10 μmol l^-1, respectively were obtained. At 102 μmol l^-1 among-series standard deviation of 6 μmol l^-1 was obtained. Comparisons between HPLC and autoanalyser (Jaffé reaction) methods showed that the autoanalyser plasma and serum results, which are known to be sensitive to ‘pseudocreatinines’, varied between 93–217% of the HPLC results. Comparisons between HPLC, autoanalyser and enzymatic methods showed that the methods agree well for urine samples. The method presented appears suitable as a secondary reference method.

Key words:

• creatinine
• enzymatic method
• HPLC
• ion-pair
• Jaffé reaction
• secondary reference method