4
Views
1
CrossRef citations to date
0
Altmetric
ORIGINAL ARTICLES

Estimation of granulocyte and lymphocyte potassium in normal subjects and in patients treated with diuretics because of cardiovascular disease: a methodological study

, &
Pages 85-91 | Received 27 Feb 1990, Accepted 12 Sep 1990, Published online: 26 Feb 2018
 

Abstract

Melchior T, Hallam D, Pedersen NT. Estimation of granulocyte and lymphocyte potassium in normal subjects and in patients treated with diuretics because of cardiovascular disease: a methodological study. Scand J Clin Lab Invest 1990; 51: 85–91.

A method is described for measuring the potassium content in leukocytes using Percoli (R) density gradient centrifugation. Ninety subjects between 21 and 92 years formed the reference population. The magnesium content in leukocytes could not be estimated because of interaction between the ion and the Percoll (R) media. Sex, age, leukocytosis because of infection, physical stress, venous stasis did not interfere with the analysis. The potassium content was calculated per cell and per g-1 of DNA. The granulocyte potassium content was (median (range)) 37.4 (25.8-75.0) fmol/cell-1 or 2.9 (1.5-9.8) mmol g-1 DNA. The lymphocyte potassium content was 45.9 (26.4-69.6) fmol cell-1 or 3.3 (1.5-5.0) mmol g-1 DNA. The coefficient of variation (<10%) was not reduced by using cell DNA instead of cell number as reference. The interindividual variation was high, making the test unfit for clinical use.

The leukocyte potassium content was not decreased in patients with acute myocardial infarction nor in patients treated with diuretics and potassium supplements because of cardiovascular disease.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.