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Original Article

Changes in hematological indices and lymphocyte subsets in response to whole blood donation in healthy male donors

, , , , , , , , , , , , & show all
Pages 143-148 | Received 16 Nov 2016, Accepted 21 Jan 2017, Published online: 20 Feb 2017
 

Abstract

Whole blood donation has immunomodulatory effects, and most of these have been observed at short intervals following blood donation. This study aimed to investigate the impact of whole blood donation on lymphocyte subsets over a typical inter-donation interval. Healthy male subjects were recruited to study changes in complete blood count (CBC) (n = 42) and lymphocyte subsets (n = 16) before and at four intervals up to 106 days following blood donation. Repeated measures ANOVA were used to compare quantitative variables between different visits. Following blood donation, changes in CBC and erythropoietin were as expected. The neutrophil count increased by 11.3% at 8 days (p < .001). Novel changes were observed in lymphocyte subsets as the CD4/CD8 ratio increased by 9.2% (p < .05) at 8 days and 13.7% (p < .05) at 22 days. CD16-56 cells decreased by 16.2% (p < .05) at 8 days. All the subsets had returned to baseline by 106 days. Regression analysis showed that the changes in CD16-56 cells and CD4/CD8 ratio were not significant (Wilk’s lambda = 0.15 and 0.94, respectively) when adjusted for BMI. In conclusion, following whole blood donation, there are transient changes in lymphocyte subsets. The effect of BMI on lymphocyte subsets and the effect of this immunomodulation on the immune response merit further investigation.

Acknowledgements

The authors gratefully acknowledge funding support from the Saudi Government represented by the Saudi National Guard Health Affairs at King Abdullah International Medical Research Center – Western Region (KIMRC-WR).

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Additional information

Funding

The funding support was from King Abdullah International Medical Research Center ? Western Region (KAIMRC-WR), Saudi Arabia-Jeddah [Grant number RCJ0611-183, Aug 14th 2012].

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