Carbon monoxide reduces near-infrared spectroscopy determined ‘total’ hemoglobin: a human volunteer study

Abstract

Carbon monoxide (CO) increases middle cerebral artery mean flow velocity (MCAV_mean), but the effect of CO on the near-infrared spectroscopy (NIRS) determined cerebral oxygenation (S_cO₂) is not detailed. In our study, 11 non-smoking subjects breathed 100% O₂ through a closed circuit. A CO₂ scrubber with CO (1.5 mL kg⁻¹) was added to the circuit. Two NIRS systems (NIRO-200NX and INVOS-5100) assessed S_cO₂ as the ratio of oxygenated to deoxygenated hemoglobin, while venous blood samples were analyzed for carboxyhemoglobin (COHb). After CO/O₂ rebreathing COHb increased to 8.7% (IQR; 7.9–9.4; p = .004) vs. normoxia, but MCAV_mean remained stable (55.6 cm s⁻¹; 53.1–69.7) compared to inhalation of O₂ (54.6 cm s⁻¹; 48.4–62.9; p = .178) and normoxia (54.1 cm s⁻¹; 44.5–66.9; p = .055). Also, INVOS-5100 determined S_cO₂ increased during CO/O₂ (74.4 ± 7.5%) and O₂ inhalation (73.1 ± 7.2%) compared to normoxia (68.9 ± 6.9%; p < .001). In contrast, NIRO-200NX determined S_cO₂ remained unchanged during CO/O₂ and O₂ inhalations but oxygenated and deoxygenated hemoglobin decreased (by 19.7 μM (median; IQR 2.8–34.8; p = .016) and 37.3 μM (30.8–46.6; p = .004), respectively) during inhalation of CO/O₂ compared to inhalation of O₂. Therefore, NIRO-200NX determined ‘total’ hemoglobin (sum of O₂Hb and HHb) decreased (by 62.1 μM; 44.5–78.2; p = .001). In conclusion, exposure to CO did not increase MCAV_mean, and neither NIRO-200NX nor INVOS-5100 detected a change in S_cO₂ when CO was added to inhalation of oxygen. Unaffected S_cO₂ after exposure to CO reflected a similar decrease in oxygenated and deoxygenated hemoglobin suggesting that detection of exposure to CO by NIRS should focus on ‘total’ hemoglobin rather than on S_cO₂.

Keywords:

• Carbon monoxide
• carbon monoxide poisoning
• carboxyhemoglobin
• cerebral blood flow
• near-infrared spectroscopy
• oximetry
• transcranial Doppler ultrasonography

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.