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Original Articles

The effect of rheumatoid factor on three commercial immunoassays for serum cystatin C

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Pages 112-115 | Received 04 Oct 2020, Accepted 13 Dec 2020, Published online: 06 Jan 2021
 

Abstract

Cystatin C (CysC) can be used to diagnose early changes in renal insufficiency. However, there are little researches to study whether there is an interference between the level of CysC and RF. Thus, we conducted this study to investigate it. We randomly selected 30 patients with high RF (RF concentration: 552.05 ± 476.23 IU/mL) and 33 healthy subjects with RF concentration <11.1 IU/mL and CysC were measured with different reagents and instruments; Interference experiment was also be included. The results showed that the measured CysC concentration increases with increasing RF concentration in a dose-dependent manner and CysC levels are falsely increased by RF interference depends on the reagents used (CysC reagent: Whitman, Nanjing, China). Reagent manufacturers should fully consider RF interference when developing CysC reagents, and evaluate them before they are sold. When selecting CysC reagents, we should evaluate RF interference to the measurement to avoid misleading results.

Disclosure statement

The funding organizations played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Additional information

Funding

This study was supported by the Natural Science Foundation of Jiangsu Province of China [BK20181492], the National Key Clinical Department of Laboratory Medicine of China in Nanjing, Key laboratory for Laboratory Medicine of Jiangsu Province [ZDXKB2016005] and by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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