Reference change values of FIB-4

Abstract

When comparing two analytical results for the same analyte, the clinicians may benefit from knowing the reference change values (RCVs) of the analyte. For Fibrosis-4 Index (FIB-4), a noninvasive test used for assessing the risk of liver fibrosis, no RCVs have been published for non-cirrhotic individuals. Therefore, we estimated RCVs for adults, using retrospectively collected data from outpatients with AST, ALT, and thrombocytes within the respective reference intervals. FIB-4 was calculated as (age × AST)/(thrombocytes × ALT^0.5). From two FIB-4 values in each patient we calculated the RCVs parametrically and non-parametrically. For both methods, we estimated the limits of the central 90% of the distribution of the ratio between the second and the first measurement. We obtained data on 599 outpatients with two blood tests taken 3 - 972 (median 258) days apart. The RCVs were 0.72 − 1.40 and 0.72 − 1.43, respectively, using the parametric and non-parametric methods. The 5 and 95 percentiles were not statistically significantly associated with sex, age, level of analyte, or the time between the measurements. The within-subject biological variation of FIB-4 was estimated to be 13.9%. Conclusion: In 90% of the patients the ratio between the second and the first FIB-4 result was approximately 0.7 − 1.4.

Keywords:

• Biological variation
• population
• fatty liver
• liver cirrhosis
• liver fibrosis
• liver function tests

Disclosure statement

No potential conflict of interest was reported by the authors.