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Abstract
A micromethod for the determination of calcium and magnesium in 0,05 ml of serum by titration with ethylene-diamine-tetra-cetate and the dye Eriochrome Black T as indicator is described. The sum of calcium and magnesium is found by direct titration of serum. Calcium is determined after precipitation as oxalate. Perchloric acid is used for decomposition of oxalate, whereby calcium becomes directly titratable. Magnesium represents the difference between the two titrations.
The method has shown sufficient accuracy, having a mean of deviations for calcium of ± 1 per cent and for magnesium of ± 5 per cent.