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Abstract
The amount of free hydroxyproline in serum from ten men in the postabsorbtive state was recorded using a bed of Zeocarb for the absorbtion of the amino acids with subsequent elution with ammonia and chromatographic fractionation on a Dowex 50 column. The hydroxyproline eluted was estimated according to Stegeman. The amount of free hydroxproline found in serum of this group of men varies from 0.07 to 0.12 mg per 100 ml.